Dr. N. Appala Raju

Dr. N. Appala Raju (Appala Raju Nemala) is a Professor in the Department of Pharmacognosy & Phytochemistry at Sultan-ul-Uloom College of Pharmacy, Hyderabad, with more than 21 years of teaching and research experience across pharmaceutical analysis, pharmacognosy, and pharmacology. He holds a Ph.D. in Pharmaceutical Analysis from JNTU Hyderabad and has authored over 70 research publications in national and international journals, alongside two granted patents and book chapters currently under review. His research expertise centres on HPLC, UPLC, and LC-MS/MS method development and validation, separation sciences, bioanalysis, and stability and dissolution studies for pharmaceutical and nutritional products. He has served as Head of Content Development for GPATtutor.com and FPGEEtutor.com, developed the curriculum for the PMKVY-sponsored Drug Regulatory Affairs Chemist Program, and currently serves as Examiner-in-Chief for undergraduate and postgraduate examinations at Sultan-ul-Uloom College of Pharmacy.

• Ph.D. in Pharmaceutical Analysis — Jawaharlal Nehru Technological University (JNTUH), Hyderabad (2010)
• M.Pharmacy (Pharmacognosy & Phytochemistry) — Andhra University, Visakhapatnam (2001)
• B.Pharmacy — Andhra University, Visakhapatnam (1999)
• Diploma in Pharmacy — Govt. Polytechnic College, Visakhapatnam (1994)

Selected from 70+ peer-reviewed publications

• N. Appala Raju, et al. “Synthesis of azachalcones, their α-amylase, α-glucosidase inhibitory activities, kinetics, and molecular docking studies.” Bioorganic Chemistry, Vol. 106, January 2021.
• N. Appala Raju, et al. “Identification, synthesis, and characterization of β-isomer as a process-related impurity in the production of methocarbamol from guaifenesin.” Journal of Applied Pharmaceutical Sciences, Vol. 10(5), May 2020.
• N. Appala Raju, et al. “Inhibitory Activities of α-Glucosidase and α-Amylase and their Hypoglycaemic Capability in the Treatment of Diabetes.” EC Pharmacology and Toxicology, 7.2 (2019).
• N. Appala Raju, et al. “Visible Extractive Spectrophotometric Estimation of Sofosbuvir in Bulk and in Pharmaceutical Formulations.” Research Journal of Pharmacy and Technology, 12(4):1517, January 2019.
• N. Appala Raju, et al. “LC Method Development and Validation for the Determination of Ropivacaine Hydrochloride in Bulk Drug and Pharmaceutical Formulations.” Pharmaceutical Chemistry Journal, 51(1):1-10, 2018.
• P. Govardhan Reddy, V. Kiran Kumar, VVSS. Appala Raju, J. Raghu Ram, N. Appala Raju. “The Estimation of Ceftaroline Fosamil in Lyophilized Powder for Injection by RP-HPLC.” Research Journal of Pharmacy and Technology, 11(2): 455-458, 2018.
• Dr. Macharla Venkata Ramana, Dr. T. Shyam, K. N. Rajinikanth, Dr. V. Kiran Kumar, Dr. VVSS. Appala Raju, Dr. N. Appala Raju. “Simultaneous Estimation of Carbodenafil and Desmethyl Carbodenafil from Human Plasma by Liquid Chromatography-Mass Spectrometry.” World Journal of Pharmaceutical Research, 7(19), 2018.
• Dhananjaya G., et al. “Development and Validation of a Stability-Indicating RP-HPLC Method for the Determination of Bendamustine in Raw Material and Finished Product.” World Journal of Pharmaceutical Research, 2018.
• Dr. VVSS. Appala Raju, Dr. Macharla Venkata Ramana, Dr. T. Shyam, K.N. Rajinikanth, Dr. V. Kiran Kumar, Dr. N. Appala Raju. “Bioanalytical Method Development and Validation for Simultaneous Determination of Bictegravir, Tenofovir and Emtricitabine in Human Plasma by LC-MS/MS.” World Journal of Pharmacy and Pharmaceutical Sciences, 2018.
• Dr. T. Shyam, K.N. Rajinikanth, Dr. V. Kiran Kumar, Dr. VVSS. Appala Raju, Dr. Macharla Venkata Ramana, Dr. N. Appala Raju. “LC-MS/MS Method Development and Validation for Simultaneous Quantification of Elbasvir and Grazoprevir in Human Plasma.” European Journal of Biomedical and Pharmaceutical Sciences, 2018.
• N. Appala Raju, et al. “New RP-HPLC Method Development and Validation for the Determination of Pazopanib Hydrochloride in Tablet Dosage Form.” International Journal of Pharmaceutical Quality Assurance, 8(1), January 2017.
• N. Appala Raju, et al. “A Validated RP-HPLC Method for the Determination of 2-Chloroadenosine as Process Related Impurity in Regadenoson Parenteral Dosage Form.” Journal of Chemical and Pharmaceutical Research, 9(7):55-61, 2017.
• Raju N. Appala, Sridevi Chigurupati, Raju V.V.S.S. Appala, Kesavanarayanan Krishnan Selvarajan, Jahidul Islam Mohammad. “A Simple HPLC-UV Method for the Determination of Glutathione in PC-12 Cells.” Scientifica, Vol. 2016, Article ID 6897890.
• N. Appala Raju, et al. “New High Performance Liquid Chromatographic Method for Determination of Vilazodone in Pharmaceutical Dosage Forms.” European Journal of Pharmaceutical and Medical Research, 3(1), 341-346, 2016.
• Dr. Nemala Appala Raju, et al. “New High Performance Liquid Chromatographic Method for Determination of Opipramol in Pharmaceutical Dosage Forms.” Indo American Journal of Pharmaceutical Research, 2016.
• Ravi Pratap Pulla, B.S. Sastry, Y. Rajendra Prasad, N. Appala Raju, et al. “Simultaneous Estimation of Metformin HCl and Sitagliptin Phosphate in Tablet Dosage Forms by RP-HPLC.” Research Journal of Pharmacy and Technology, 4(4): 646-649, 2011.
• N. Appala Raju, J. Venkateswara Rao, K. Vanitha Prakash, K. Mukkanti, K. Srinivasu, et al. “Simultaneous Estimation of Tenofovir Disoproxil, Emtricitabine and Efavirenz in Tablet Dosage Forms by RP-HPLC.” Oriental Journal of Chemistry, 24(2): 645, 2008.
• K. Vanitha Prakash, J. Venkateswara Rao, N. Appala Raju, et al. “RP-HPLC Method for the Estimation of Nelfinavir Mesylate in Tablet Dosage Form.” E-Journal of Chemistry, 4(3): 302, 2007.
• Nemala Appala Raju, et al. “Novel Acetyl Cholinesterase Inhibitors to Treat Neuro-degenerative Disorders.” Provisional Patent, Intellectual Property India, Application No. 201741009498.
• Nemala Appala Raju, et al. “Simultaneous Assessment of Eight-DPPH Inhibitors Gliptins and Metformin by HPLC.” Provisional Patent, Intellectual Property India, Application No. 201941035153.

• HPLC, UPLC & LC-MS/MS method development and validation
• Bioanalytical method development for pharmacokinetic studies
• Pharmaceutical impurity profiling & process-related impurities
• Separation sciences and stability/dissolution studies
• Computer-aided drug design (CADD)